A patch-clamp study of histamine-secreting cells.
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منابع مشابه
A patch-clamp study of histamine-secreting cells
The ionic conductances in rat basophilic leukemia cells (RBL-2H3) and rat peritoneal mast cells were investigated using the patch-clamp technique. These two cell types were found to have different electrophysiological properties in the resting state. The only significant conductance of RBL-2H3 cells was a K+-selective inward rectifier. The single channel conductance at room temperature increase...
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Pancreatic α-cells secrete glucagon to maintain energy homeostasis. Although histamine has an important role in energy homeostasis, the expression and function of histamine receptors in pancreatic α-cells remains unknown. We found that the histamine H3 receptor (H3R) was expressed in mouse pancreatic α-cells and αTC1.6 cells, a mouse pancreatic α-cell line. H3R inhibited glucagon secretion from...
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Ion channels are integral membrane proteins that regulate the flow of ions across the plasma membrane and the membranes of intracellular organelles of both excitable and non-excitable cells. Ion channels are vital to a wide variety of biological processes and are prominent components of the nervous system and cardiovascular system, as well as controlling many metabolic functions. Furthermore, i...
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Christian Baum ann Physiologisches Institut der Justus-Liebig-Universität Gießen, Aulweg 129, D-6300 Gießen, Bundesrepublik Deutschland Z. Naturforsch. 40c, 886—890 (1985); received October 23, 1985 Patch Clamp, Membrane ATPase, Tonoplast, Vacuole, Chenopodium rubrum L. The patch clamp technique has been applied to isolated vacuoles from green suspension cells of Chenopodium rubrum to record el...
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Due to its exquisite sensitivity and the ability to monitor and control individual cells at the level of ion channels, patch-clamping is the gold standard of electrophysiology applied to disease models and pharmaceutical screens alike. The method traditionally involves gently contacting a cell with a glass pipette filled by a physiological solution in order to isolate a patch of the membrane un...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1986
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.88.3.349